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. 2022 Jan 7;12(5):2330–2347. doi: 10.1016/j.apsb.2022.01.001

Figure 3.

Figure 3

Reduction of neural excitability in Trpv3−/− striatal medium spiny neurons. (A) Morphology of a typical mouse medium spiny neuron (MSN) in the striatum labeled with neurobiotin. (B) Representative traces for neuronal firings by whole-cell current-clamp recordings of the MSN at resting membrane potential (RMP) from Trpv3+/+ and Trpv3−/− mice. (C) The summary of action potential numbers (AP No.) at RMP. AP No. of each current step was analyzed by two-tailed nonparametric Mann–Whitney test. (D) The summary of RMP values. (E) Representative traces for neuronal firings of MSNs at −80 mV from Trpv3+/+ and Trpv3−/− mice. (F) The summary of AP No. at −80 mV. AP No. of each current step was analyzed by two-tailed nonparametric Mann–Whitney test. (G) Representative traces for mEPSCs in striatal MSNs from Trpv3+/+ and Trpv3−/− mice by whole-cell voltage clamp recordings. (H) The frequency of mEPSCs and associated cumulative probability (I). (J) Left panel, representative images showing the morphology of the spines in striatal MSNs from Trpv3+/+ and Trpv3−/− mice. The summary of dendrite spine density (middle panel) and a schematic drawing of dendrite branch classifications (right panel). Data are expressed as mean ± SEM, n represents the number of neurons patched. Two-way ANOVA was used and followed by Holm–Sidak's test from GraphPad 8. ∗P < 0.05, ∗∗P < 0.01 and ∗∗∗P < 0.001. Also see Fig. S2 for no obvious morphological changes of complex dendritic trees in Trpv3+/+ and Trpv3−/− neurons; Fig. S3 for reduced neural excitability of cortical stellate neurons from Trpv3−/− mice and Table S1 for electrophysiological parameters on medium spiny neurons (MSNs) and layer II principal stellate neurons from medial entorhinal cortex (mEC) from Trpv3+/+ and Trpv3−/− mice and Table S2 for mEPSCs and mIPSCs parameters of mouse striatal MSNs or mEC layer II stellate neurons.