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. 2021 Dec 22;12(5):2494–2505. doi: 10.1016/j.apsb.2021.12.010

Figure 2.

Figure 2

T17 activates the STING/IRF3/IFN-I pathway in macrophages and favors M1 polarization. After treatment of TDNs (200 nmol/L) in different sizes with optiMEM for 3 h, cells were cultured with complete culture medium and collected at 24 h. (A) The mRNA level of Ifnb in BMDMs was detected by Real-time PCR (B) and the protein levels of TBK1, IRF3 and INOS in BMDMs were detected by Western blot. After treatment of T17 (200 nmol/L), (C) cell viability of BMDMs was detected by CCK-8 assay, (D) the mRNA levels of the genes in BMDMs were detected by Real-time PCR and displayed by heat map, (E) the protein levels of INOS and ARG-1 in BMDMs were detected by Western blot, (F) and the protein level of INOS was detected by flow cytometry. (G) After treatment of T17 (200 nmol/L), the mRNA level of Inos in WT or STING KO (Tmem173−/−) BMDMs was detected. (H, I) After 3 h incubating with Cy5-a, Cy5-ab or Cy5-abc, the mRNA level of Ifnb and Inos was detected by Real-time PCR. Data are presented as mean ± SD (n = 3). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001, ns, not significant.