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. 2021 Dec 22;12(5):2494–2505. doi: 10.1016/j.apsb.2021.12.010

Figure 3.

Figure 3

T17 and manganese ion synergistically enhances the immune response. After treatment of different minerals and T17 (200 nmol/L) for 24 h, (A–C) the mRNA levels of Ifnb, Isg15, Inos in BMDMs were detected by Real-time PCR (D, E) and the protein levels of INOS, the phosphorylation of TBK1, IRF3 and STING, the dimerization of STING was detected by Western blot. (F–I) BMDMs were pretreated with T17 (200 nmol/L) and MnCl2 (50 μmol/L) for 24 h (pt-BMDMs), followed by co-culture with T cells for 3 days, or T cells were treated by T17 (200 nmol/L) and MnCl2 (50 μmol/L) directly and then co-cultured with BMDMs for 3 days (without pt-BMDMs), CD69 and IFN-γ in CD4+ T cells, as well as CD69 and granzyme B in CD8+T cells were detected by flow cytometry. Data are presented as mean ± SD (n = 3). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001, ns, not significant.