The in vivo biodistribution and pharmacokinetic profiles of NGP@MI. (A) The in vivo distribution images of free DIR and DIR loaded in NG@M (NGDIR@M) at different time points (n = 3, representative images). (B) The fluorescence images of major organs at different time points after i.v. administration of free DIR and NGDIR@M (n = 3, representative images). Abbreviations: T, tumor; H, heart; Li, liver; S, spleen; Lu, lung; Ki, kidney. Quantification of fluorescence intensity of major organs at different time points after i.v. administration of free DIR (C) and NGDIR@M (D) (n = 3). Pharmacokinetic profiles of PTX (E) and IL-2 (F) after i.v. injection of free PTX + IL-2 and NGP@MI (n = 3).