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. 2021 Nov 9;12(5):2550–2567. doi: 10.1016/j.apsb.2021.11.004

Figure 4.

Figure 4

The immunogenic cell death effect of NGP@M. (A) CRT expression of 4T1 cancer cells treated with saline, NG@M, free PTX (Cremophor EL formulation) and NGP@M after 12 h (red: CRT marker, green: cell membrane labeled with DIO, blue: nuclei labeled with DAPI, scale bar = 10 μm) (B) The illustration of ICD effect. (C) The proportion of CRT expressed in living 4T1 cancer cells (represented as CRT+/PI−) at 12 h post-treatment with saline, NG@M, free PTX and NGP@M (n = 3). The levels of ATP (D) and HMGB-1 (E) released from 4T1 cancer cells at 24 h post-treatment with saline, NG@M, free PTX and NGP@M (n = 3). The quantitative proportion (F) and scatter plots (G) of matured BMDCs at 24 h post-treatment with LPS, saline, and 4T1 cancer cells which were pre-treated with saline, NG@M, PTX or NGP@M. Data are presented as mean ± SD (n = 3). Statistical P values: ∗∗P < 0.01, ∗∗∗P < 0.001.