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. 2021 Oct 28;12(5):2506–2521. doi: 10.1016/j.apsb.2021.10.022

Figure 4.

Figure 4

Intracellular behavior and biological effects of anti-angiogenesis and anti-inflammation of MRaNPs in ARPE-19 cells. (A) CLSM images of the intracellular behavior of MRaNPs (blue: DAPI for nucleus; green: LAMP1 for lysosome; red: DiI-labeled MRaNP). Scale bar = 10 μm. (B) WB analysis for VEGFA and the mTOR signaling pathway. (C) CLSM images of autophagy induction stained with AO (green: nucleus; red: autolysosome). Scale bar = 20 μm. (D) CLSM images of autophagic flux (blue: DAPI for nucleus; green: LAMP1 for lysosome; red: LC3 for autophagosome). Scale bar = 20 μm. (E to F) Expression of cytokine IL-6 and IL-8 measured by enzyme-linked immunosorbent assay (n = 3, ∗∗P < 0.01, ∗∗∗P < 0.001 and ∗∗∗∗P < 0.0001). (G) WB analysis for autophagy-associated proteins.