Table 4.
Primers used for the quantitative RT-PCR (qPCR).
| Gene name | Primer sequence (5′-3′) |
|---|---|
| Relative gene expressions of the GH/IGF-1 axis and TOR signal pathway in the liver | |
| GHa | F:CCCCCAAACTGTCAGAACT R:ACATTTCGCTACCGTCAGG |
| IGF-1b | F: CTTCAAGAGTGCGATGTGC R:GCCATAGCCTGTTGGTTTACTG |
| TORc | F:TCAGGACCTCTTCTCATTGGC R:CCTCTCCCACCATGTTTCTCT |
| PI3Kd | F:AAGACCTTCCTCATCACGAC R:CCTTCCACTACAACACTGCA |
| S6Ke | F:GCCAATCTCAGCGTTCTCAAC R:CTGCCTAACATCATCCTCCTT |
| Immune Response Related Gene Expression in Intestine | |
| SODf | F:TGGCAAGAACAAGAACCACA R:CCTCTGATTTCTCCTGTCACC |
| GSHPxg | F:GGGGCTCCACCTGCTTCTTG R:ACCCCTCTGCTCAGGCATTT |
| IL1βh | F:CGTGACTGACAGCAAAAAGAGG R:GATGCCCAGAGCCACAGTTC |
| IL15i | F:GTATGCTGCTTCTGTGCCTGG R:AGCGTCAGATTTCTCAATGGTGT |
| TNFβj | F:GCTCAAAGAGAGCGAGGATG R:TCCTCTACCATTCGCAATCC |
| Caspase 3 | F:GCTTCATTCGTCTGTGTTC R:CGAAAAAGTGATGTGAGGTA |
| Caspase 9 | F:CTGGAATGCCTTCAGGAGACGGG R:GGGAGGGGCAAGACAACAGGGTG |
| Caspase 10 | F:CAAACCACTCACAGCGTCTACAT R:TGGTTGGTTGAGGACAGAGAGGG |
| Relative peptide and AA transporters | |
| SLC7A5k | F:CCAAAGCACGACAGACCTACA R:ACCAACCTGGCATATTTCACC |
| SLC7A8l | F:GGTGACCACAGGGATAGAGATG R:TTGCTTACGGAGGCTGGAACTT |
| SLC38A2m | F:AATAGGGAAAAGCACCACGGG R:GTATGAGGAGCTCAAAGACCG |
| SLC15A1 (PepT1)n | F:AAAGCAGGCAGCACCTTCACTC R:CCTCTCGCAGAACTCATTCACAAC |
| SLC15A2o | F:TGCACATCCCCTCTCAGTACG R:CAAGTCAGTTGGAGCCATTCC |
| β-actin | F:ATCGCCGCACTGGTTGTTGAC R:CCTGTTGGCTTTGGGGTTC |
aGH: Growth hormone; bIGF-1: Insulin growth factor 1 (IGF-1); cTOR: target of rapamycin; dPI3K: phosphatidylinositol 3-hydroxy kinase; eS6K: S6 Kinase; fSOD: superoxide dismutase; gglutathion peroxidase; hIL1β: interleukin 1β; iIL15: interleukin 15; jTNF β: tumor necrosis factor β; kSLC7A5: solute carrier family 7, member 5; lSLC7A8: solute carrier family 7, member 8; mSLC38A2: solute carrier family 38, member 2; nSLC15A1 (PepT1): solute carrier family 15, member 1, oligopeptide transporter 1, PepT1; oSLC15A2: solute carrier family 15, member 2, oligopeptide transporter 2, PepT2.