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. 2022 May 13;25(6):104405. doi: 10.1016/j.isci.2022.104405

Figure 2.

Figure 2

PMS effectively promoted the proliferation and differentiation of NPMSCs

(A) The CCK-8 assay results showed that the rate of proliferation was significantly higher in the PMS group than in the MCDM group on days 5 and 7, and the rate of proliferation was significantly increased when MCDM was combined with PMS. Results are presented as the mean ± SD of three independent experiments (∗p < 0.05, ∗∗∗p < 0.001).

(B) The levels of COL 2A1 and ACAN mRNA expression in the MCDM, PMS, and MCDM + PMS groups were detected by quantitative RT–PCR. The results showed that the mRNA expression of COL 2A1 and ACAN was significantly increased in the MCDM + PMS group compared with that in two other groups. Results are presented as the mean ± SD of three independent experiments (∗p < 0.05, ∗∗p < 0.01). (A–B) MCDM was used as an internal control.

(C–F) The protein levels of COL 2A1, ACAN, and BMP-2 were analyzed by western blotting (C), and the relative quantitative data (D–F) were calculated. GAPDH was used as an internal control. Results are presented as the mean ± SD of three independent experiments (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).