Figure 5.

PMS promoted the proliferation and differentiation of NPMSCs through activation of the BMP-2/Smad1/Runx2 axis by ITG A1

(A–H) The protein levels of Smad1, RUNX2, ITG A1, BMP-2, COL 2A1, and ACAN were analyzed by western blotting (A, C, E, G), and the relative quantitative data (B, D, F, H) were calculated. GAPDH was used as an internal control. (A–B) BMP-2 + PMS effectively promoted the expression of SMAD1 and Runx2. Results are presented as the mean ± SD of three independent experiments (∗p < 0.05). (C–D) The knockdown reagent effectively reduced the expression of ITG A1. Results are presented as the mean ± SD of three independent experiments (∗∗∗p < 0.001). (E–F) The expression of all proteins was decreased when ITG A1 was knocked down. Results are presented as the mean ± SD of three independent experiments (∗∗∗p < 0.001). (G–H) The protein expression of ITG A1 was not influenced by the knockdown of BMP-2, and the expression of other proteins was decreased. Results are presented as the mean ± SD of three independent experiments (∗p < 0.05, ∗∗∗p < 0.001).