FIGURE 6.

Representative confocal images and 3D reconstructions of stellate ganglia (SG) sympathetic neurons. (a): Confocal image of a cardiac‐projecting SG neuron (scale bar =15 µm). (b): Confocal image of a BAT‐projecting SG neuron (scale bar =20 µm). A region of interest and selective masking was used to isolate single neurons in a confocal scan for analysis. (c): A 3D reconstruction of the cardiac‐projecting neuron (a); (d): 3D reconstruction of the BAT‐projecting neuron (b). (c and d): Imaris Surfaces‐ and Filaments‐generated objects modeled soma and dendrites. Each primary dendrite branch point is labeled blue. The soma volume (µm³) and number of primary dendrites of each cell was assessed. (e): A confocal image of a cardiac‐projecting SG neuron. (f): A confocal image showing vesicular acetylcholine transporter (VAChT) puncta in SG. (g): Imaris reconstructions of a cardiac‐projecting neuron and VAChT puncta (white spots) that were apposed to the soma surface. Scale bars for E, F, and G = 10 µm.