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. 2021 Dec 1;3(4):687–699. doi: 10.34067/KID.0004572021

Figure 2.

Figure 2.

Effects of UBE-1099 on renal dysfunction in an Alport mouse model. (A) and (B) Urine samples were collected at the indicated time points. Urinary protein and albumin were measured using the Bradford and ELISA methods, respectively. Urinary creatinine was measured using Jaffe’s method. Urinary protein and albumin concentration were normalized with urinary creatinine concentration and presented as proteinuria and albuminuria, respectively. (C) GFR was measured with a transdermal GFR monitor (MediBeacon) in 22-week-old wild-type (WT) and Alport mice. (D) and (E) Plasma creatinine and BUN were measured using DRI-CHEM (Fujifilm) and a 7180 biochemistry automatic analyzer (Hitachi), respectively, in 22-week-old WT and Alport mice. (F) Total RNA was isolated from the renal tissue of 22-week-old mice. The level of the Lcn2 mRNA was measured and normalized to the level of Gapdh mRNA (internal control). Data are presented as the mean±SEM (n=7–8 per group). P values were assessed by Dunnett’s test. *P<0.05, **P<0.01, ***P<0.001.