Figure 5.

UBE-1099 induced Nrf2 activation and ameliorated the dysfunction of Nrf2 signaling in the renal tissue of an Alport mouse model. (A) Whole cell protein and (B) nuclear fraction protein were isolated from kidney tissue in the indicated group at 22 weeks of age and were analyzed by immunoblotting. The full-length blots are presented in Supplemental Figure 13. (C–E) The relative amount of proteins was quantified using Image Gauge software (Fujifilm) and normalized with Vinculin and HDAC2 (internal control). Data are presented as the mean±SEM (n=3–4 per group). P values were assessed by Dunnett’s test. *P<0.05, **P<0.01, ***P<0.001. (F–H) Total mRNA was isolated from renal tissues of 22-week-old WT or Alport mice. The level of the indicated mRNA was normalized to the level of Gapdh mRNA (internal control). Data are presented as the mean±SEM (n=7–8 per group). P values were assessed by Dunnett’s test. ***P<0.001.