Table 1.
Primers used in quantitative RT-PCR
| Gene | Sense | Antisense |
|---|---|---|
| Nqo1 a | 5′-CAGCCAATCAGCGTTCGGTA-3′ | 5′-CTTCATGGCGTAGTTGAATGATGTC-3′ |
| Gapdh a | 5′-TGTGTCCGTCGTGGATCTGA-3′ | 5′-TTGCTGTTGAAGTCGCAGGAG-3′ |
| Lcn2 | 5′-CAGAAGGCAGCTTTACGATG-3′ | 5′-CCTGGAGCTTGGAACAAATG-3′ |
| Kim1 | 5′-GGAAGTAAAGGGGGTAGTGGG-3′ | 5′-AAGCAGAAGATGGGCATTGC-3′ |
| Tgf-b | 5′-CACCTGCAAGACCATCGACAT-3′ | 5′-GAGCCTTAGTTTGGACAGGATCTG-3′ |
| IL-1b | 5′-GCTGAAAGCTCTCCACCTCAATG-3′ | 5′-TGTCGTTGCTTGGTTCTCCTTG-3′ |
| Mcp1 | 5′-GAAGCTGTAGTTTTTGTCACCAAG-3′ | 5′-AGGTAGTGGATGCATTAGCTTCA-3′ |
| Tnfa | 5′-CATCTTCTCAAAATTCGAGTGACAA-3′ | 5′-TGGGAGTAGACAAGGTACAACCC-3′ |
| Mmp9 | 5′-GGACCCGAAGCGGACATTG-3′ | 5′-CGTCGTCGAAATGGGCATCT-3′ |
| Il-6 | 5′-GAGGATACCACTCCCAACAGACC-3′ | 5′-AAGTGCATCATCGTTGTTCATACA-5′ |
| Nrf2 | 5′-CACTCCAGCGAGCAGGCTAT-3′ | 5′-CTGGGACTGTAGTCCTGGCG-3′ |
| Nqo1 b | 5′-TTCTGTGGCTTCCAGGTCTT-3′ | 5′-AGGCTGCTTGGAGCAAAATA-3′ |
| Gclc | 5′-GGACAAACCCCAACCATCC-3′ | 5′-GTTGAACTCAGACATCGTTCCT-3′ |
| Gapdh b | 5′-CCTGGAGAAACCTGCCAAGTATG-3′ | 5′-GGTCCTCAGTGTAGCCCAAGATG-3′ |