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. 2022 May 17;144(20):8945–8950. doi: 10.1021/jacs.2c03925

Figure 2.

Figure 2

S-protein proteolysis by NE renders amyloid-like fibrils. Thermostability of (A) SARS-CoV-2 S-protein, (B) NE, (C) S-protein+NE, measured by DSF. Dashed line in (C) is the mathematical sum of S-protein and NE, respectively, from (A) and (B) supporting cleavage of S-protein by NE. MALDI-ToF spectra of C18 isolated peptides of (D) S-protein, (E) NE, and (F) S-protein+NE (6 h, 37 °C). TEM micrographs of (G) S-protein alone depicting the expected trimers, (H) NE alone, and (I) S-protein+NE coincubated at pH 8.4, 24 h, 37 °C.