Figure 11.

(a) ¹H-¹⁵N HSQC spectrum of 1 mM PYP in 5 mM potassium phosphate at pH 6.2. The ¹⁵N^η–¹H^η and ¹⁵N^ϵ–¹H^ϵ correlations are indicated in red and blue, respectively. (b) ¹H-decoupled and (c) ¹H-coupled ¹⁵N^η/ϵ–¹³C^ζ correlation spectra of 3 mM PYP in 5 mM potassium phosphate at pH 6.5. The ¹⁵N^η–¹³C^ζ and ¹⁵N^ϵ–¹³C^ζ correlations are indicated in red and blue, respectively. To avoid additional signals due to the presence of ²H isotopomers, D₂O for field lock was added externally to a separate compartment of the NMR tube. The number of scans is as follows: (a) 16, (b) 128, and (c) 192. In (a) and (b), 1D traces from the 2D spectra, indicated by the broken lines, are shown. Reproduced with permission from ref (31). Copyright 2017 Wiley-VCH Verlag GmbH and Co. KGaA, Weinheim.