Table 3. Enzymes of Glycolysis, Pentose Phosphate, and Nucleotide Biosynthesis and Salvage Pathway for rNTP Synthesis.

enzymea	abbreviation	EC number	source
Gilles-Schramm-Williamson and Co-workers65,121−124
Hexokinase	HXK	2.7.1.1	Baker’s yeast
Glucose-6-phosphate isomerase	PGI1	5.3.1.9	Baker’s yeast
Glucose-6-phosphate dehydrogenase	ZWF	1.1.1.49	L. mesenteroides
Phosphogluconate dehydrogenase	GND	1.1.1.44	Torula yeast
Ribose-5-phosphate isomerase	RPI1	5.3.1.6	Spinach
Phosphoribosylpyrophosphate synthetase	PRPPS	2.7.6.1	E. coli
Adenine phosphoribosyltransferase	APRT	2.4.2.7	JM109/pTTA6
Uracil phosphoribosyltransferase	UPRT	2.4.2.9	JM109/pTTU2
Xanthine-guanine phosphoribosyltransferase	XGPRT	2.4.2.22	JM109/pTTG2
Nucleoside-monophosphate kinase	NMPK	2.7.4.4	Bovine liver
Myokinase (Adenylate kinase)	MK	2.7.4.3	Rabbit muscle
Guanylate kinase	GK	2.7.4.8	Porcine brain
3-Phosphoglycerate mutase	YIBO	5.4.2.1	Rabbit muscle
Enolase	ENO	4.2.1.11	Baker’s yeast
Pyruvate kinase	PYKF	2.7.1.40	Rabbit muscle
Glutamate dehydrogenase (NAD(P)+)	GLUD	1.4.1.3	Bovine liver
CTP synthase	CTPS	6.3.4.2	JM109/pMW5
l-Lactate dehydrogenase	LDH	1.1.1.27	Rabbit muscle
Dayie and Co-workers74,75,128
Ribokinase	RK	2.7.1.15	E. coli
Creatine kinase	CK	2.7.3.2	Chicken muscle
UMP kinase	UMPK	2.7.4.22	E. coli
Serianni and Co-workers129
Purine nucleoside phosphorylase	PNPase	2.4.2.1	E. coli
Xanthine oxidase	XO	1.1.3.22	Buttermilk
Catalase	CT	1.11.1.6	Bovine liver
Uridine phosphorylase	UPase	2.4.2.3	E. coli

^aGiven that there is overlap in the enzymes used in the methods of Schramm-Williamson and co-workers^65,121−124 and Dayie and co-workers,^74,75,128 only the unique enzymes are listed for the latter. All enzymes are commercially available except APRT, UPRT, XGPRT, CTPS, and RK.¹²⁸ These are currently only available in a few academic laboratories. At some point, these plasmids would be available at Addgene.