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Spectral data and simplified pulse sequences for three methods to set the magic angle. (a) Classic 79Br (KBr) method is shown on top, methods for angle adjustment directly on a protein sample rely either on (b) optimizing the 1H T2′ (middle) or (c) H–N J coupling spin–echo (bottom). In (b), the 1H echo delay τ1 is set close to T2′/2, whereas in (c), the 15N echo delay τ2 is set to exactly (2 JNH)−1 ≈ 10.87 ms to fully refocus 1JNH coupling.
