Figure 64.

(a) Representation of conformational dynamics in hCAII. The protein backbone is represented as a cylinder, where thickness and color (gray to red) highlight areas undergoing microsecond conformational exchange in the active site loop, assessed by ¹⁵N relaxation–dispersion experiments. (b–e) Effect of ligand binding on the exchange motions of hCAII sensed by T198 backbone and N67 side-chain ¹⁵N spin. The top row shows the free form, whereas the bottom row shows the dorzolamide-bound form. Reprinted with permission from ref (339) (copyright 2019 American Chemical Society) and from ref (27) (copyright 2021 Elsevier).