Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 27;21:120. doi: 10.1186/s12943-022-01581-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 7 — MDC1 mediates effects of AP4 on DNA repair. A Detection of MDC1 and γH2AX foci by immunocytochemistry 48 h after silencing MDC1. Scale bars: 20 μm. Foci quantification was performed with Image J software. Nuclei with over 10 foci were considered as positive. The fluorescence intensity was normalized to DAPI. Quantification of 3 fields with 120 cells in total. B MDC1 and γH2AX foci were detected by immunocytochemistry 48 h after ectopic expression of MDC1. Scale bars: 20 μm. Nuclei with over 10 foci were considered as positive. The fluorescence intensity was normalized to DAPI. Quantification of 3 fields with 120 cells in total. C β-gal staining 48 h after silencing or ectopic expression of MDC1, respectively. Quantification of 3 fields with 120 cells in total. Scale bars: 50 μm. MTT assay results 48 h after D silencing MDC1 or E ectopic expression of MDC1. Detection of F γH2AX foci by immunocytochemistry, G β-gal staining and H comet assay in DLD-1 AP4 WT1 cells 48 h after transfection. MDC1-HA was rendered non-responsive to miR-22-3p by deletion of the MDC1 3'-UTR [28]. Quantification of DNA tail moment in 10 fields with 150 cells in total. The mean + SD is provided with A-E (n = 3), F-G (n = 5) and H (n = 10) with **: p < 0.01, ***: p < 0.001, ****: p < 0.0001