Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 26;20:73. doi: 10.1186/s12964-022-00868-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 4 — D347-2761 overcame BTZ drug resistance in MM cells. A RPMI-8226 cell was dealt with 5 μM D347-2761 following 10 nM BTZ treatment and cell viability was measured by CCK8 assay. B Flow cytometry assay analysis of apoptotic cell rate in RPMI-8226 cells following 5 μM D347-2761 and/or 10 nM BTZ treatment. C RPMI-8226/BTZ100 cell was treated by different dose of D347-2761 (5 μM, 10 μM, 20 μM and 30 μM) and cell viability was measured by CCK8 assay. D-E. Flow cytometry assay analysis of apoptotic cell rate in RPMI-8226/BTZ100 cells following different concentration of D347-2761 (5 μM, 10 μM and 15 μM) for 48 h, or treated by 5 μM D347-2761 and/or 10 nM BTZ treatment. F Western blot analysis of γH2AX expression in RPMI-8226/BTZ100 cells following different dose of D347-2761 or BTZ and combination (10 μM D347-2761 and 10 nM BTZ). β-actin was used to be internal control. Error bars: mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001