Fig. 1.

Effects of intermittent fasting on ICH-induced brain injury and neurological deficits in mice. Cylinder test A and corner test B tests were performed pre-ICH (n = 41–42) and at day 1 (n = 37–38), 3 (n = 27), 7 (n = 18) and 28 (n = 9) after ICH in mice with or without IF treatment. C Representative HE staining images of the largest clot at day 1, 3 and 7 days after ICH with or without IF treatment, Scale bar = 1 mm. Quantification of hematoma size D and brain volume E in ICH and ICH + IF groups (n = 6). F Examples of DARPP-1 immunohistochemistry in the basal ganglia at 28 day after ICH with or without IF treatment, Scale bar = 1 mm. G Quantification of ipsilateral shrinkage of the DARPP-32 positive area in the mice (n = 6). H TUNEL staining of ipsilateral mice brain and I the assessment of the number of TUNEL positive cells (n = 6), Scale bar = 50 μm. J Nissl staining of ipsilateral mice brain and K the assessment of the number of Nissl positive cells (n = 6), Scale bar = 50 μm. Statistical analysis was performed using two-way ANOVA analysis plus post hoc Bonferroni’s test for behavioural data (A, B), one-way ANOVA followed by post hoc Tukey’s test for multiple comparisons (D-K). Values are mean ± SD, *P < 0.05 compared with ICH group