FIG. 2.

Degradation of xanthan by extra- and intracellular enzymes of Bacillus sp. strain GL1. Lane 1, xanthan; lane 2, xanthan depolymerization products by extracellular enzymes. The degradation of the mixture of P2 and P3 (tetrasaccharides) by intracellular enzymes of Bacillus sp. strain GL1 is also shown. The tetrasaccharides were incubated at 30°C with intracellular enzymes. Samples of 10 μl were periodically removed from the reaction mixture and analyzed by TLC. Lane 3, tetrasaccharide (mixture of P2 and P3). The reaction times were as follows: lane 4, 0 min; lane 5, 40 min; lane 6, 2 h; and lane 7, 12 h. Lanes Glc, Man, and GlcA represent authentic d-glucose, d-mannose, and d-glucuronic acid, respectively.