TABLE 3.
Substrate specificity of β-d-glucanase
| Substrate | Activitya (%) |
|---|---|
| Modified xanthanb | 100 |
| Xanthan | 8.2 |
| Carboxymethyl cellulose | 1.6 |
| Lichenan [β (1,3-1,4)-glucan] from Cetraria islandica | NDc |
| β-Glucan [β (1,3-1,4)-glucan] from barley | 1.0 |
| Xylan [β (1,4)-Xylose]n from oat spelt | ND |
| pNP-β-d-Glc | ND |
| pNP-β-d-Cellobiose | ND |
| pNP-β-d-Cellotriose | ND |
| pNP-β-d-Cellotetraose | ND |
a
Reactions were carried out for 30 min at 30°C and pH 6.0 (sodium acetate buffer). Activity toward modified xanthan was taken as 100%.
b
Modified xanthan was prepared from xanthan by treatment with xanthan lyase.
c
ND, not detected.