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. 2022 May 12;13(2):19476035221077401. doi: 10.1177/19476035221077401

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© The Author(s) 2022

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — USC-EVs promoted the chondrocytes proliferation and migration. (A) TEM scanning of the USC-EVs. (B) EV surface biosignatures CD63, TSG101, Syntenin, and Calnexin, were detected by western blot. (C) The Chondrocytes uptake USC-EVs. (D) CCK-8 assay detection of the cell viability of chondrocytes. (E) EdU staining detection of the proliferation of chondrocytes. (F) Transwell assay was applied to detect the migration ability of chondrocytes. USCs = urine-derived stem cells; EVs = extracellular vehicles; TEM = transmission electron microscopy; CCK-8 = cell counting kit-8; EdU = 5-Ethynyl-2’-Deoxyuridine; DAPI = 4’,-6-diamidino-2-phenylindole. *P < 0.05, ** P < 0.01, compared with the PBS group.