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. 2022 May 12;13(2):19476035221077401. doi: 10.1177/19476035221077401

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© The Author(s) 2022

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 4. — miR-26a-5p is transferred to chondrocytes through USC-EVs. (A) Expression of miRNAs in nor-EVs and hypo-EVs was detected by qRT-PCR. (B) The expression of miR-26a-5p after transfection was assessed by qRT-PCR in USCs. (C) The expression of miR-26a-5p in EVs. (D) The expression of miR-26a-5p in chondrocytes after treatment. (E-F) Detection of cell proliferation using CCK-8 and EdU staining assays. (G) Transwell assay detection of cell migration. USCs = urine-derived stem cells; EVs = extracellular vehicles; NC = negative control; qRT-PCR = quantitative real-time polymerase chain reaction; CCK-8 = cell counting kit-8; EdU = 5-Ethynyl-2’-Deoxyuridine; DAPI = 4’,-6-diamidino-2-phenylindole; PBS = phosphate-buffered saline. *P < 0.05, **P < 0.01, compared with the PBS group; ##P < 0.01, compared with the hypo-EVs (inhibitor-NC) group.