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. 2022 May 12;13(2):19476035221077401. doi: 10.1177/19476035221077401

Figure 5.

Figure 5.

miR-26a-5p targeted PTEN in chondrocytes. (A) Prediction of miR-26-5p targets using TargetScan, miRDB, DIANA, and miRanda. (B) PTEN binding sites with miR-26-5p. (C) Dual-luciferase reporter assay confirmed the target relationship between miR-26a-5p and PTEN. (D) miR-26-5p expression in chondrocytes detected by qRT-PCR. (E-F) PTEN expression in chondrocytes after transfection. miRDB = MicroRNA Target Prediction Database (mirdb.org); DIANA = DIANA-microT-CDS (www.microrna.gr/microT-CDS), TargetScan = TargetScan Database (targetscan.org); PTEN = phosphatase and tensin homolog; WT = wild-type; MUT = mutant type; NC = negative control; qRT-PCR = quantitative real-time polymerase chain reaction. *P < 0.05, **P < 0.01.