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. 2022 Mar 7;13(1):19476035221081469. doi: 10.1177/19476035221081469

Figure 1.

Figure 1.

FR-β surface expression and other characteristics of macrophage subtypes. Experimental conditions consist of GM-CSF (black bars) or M-CSF (white bars) generated macrophages, stimulated for 24 hours with corresponding cytokine(s). (A-D) Mean fluorescence intensity (MFI) of FR-β, CD163, and CD80 as measured by flow cytometry. (E-G) Relative gene expression of FOLR2, IL10, and TGFBI, calculated using delta Ct method and housekeeping genes TBP and YWHAZ. (H-J) Cytokine concentration of TNF-α, IL-10, and IL-6 in culture supernatants measured by ELISA. Graphs represent mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 compared with GM-CSF control; &P < 0.05, &&P < 0.01, &&&P < 0.001 compared with M-CSF control, as determined by generalized linear mixed models with Bonferroni correction. FR-β = folate receptor beta; GM-CSF = granulocyte-macrophage colony-stimulating factor; M-CSF = macrophage colony-stimulating factor; TGFBI = transforming growth factor beta induced; TNF = tumor necrosis factor; IL = interleukin; ELISA = enzyme-linked immunosorbent assay.