Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 19;13(2):19476035221093060. doi: 10.1177/19476035221093060

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

PMC Copyright notice

Figure 1. — Autophagy regulates exosome release by MSCs. (A) BMSCs cultured in medium; scale bars = 100 μm. (B) Immunofluorescence staining depicting LC3+ cells (green) and quantitative analysis of intensity; scale bars = 20 μm. (C, D) Western blot analysis of Beclin1, LC3I, LC3II, and β-actin expressions in BMSCs. (E) TEM depicting autophagosomes (arrows); scale bars = 1 μm. (F) TEM observation of exosomes from BMSCs; scale bars = 200 nm. (G, H) NTA of the size distribution and amount of exosomes from BMSCs. (I, J) Western blot analysis of CD63, ALIX and TSG101 expressions in BMSCs and exosomes. MSCs = mesenchymal stem cells; BMSCs = bone marrow-derived MSCs; TEM = transmission electron microscopy; NTA = nanoparticle tracking analysis; DAPI: diamidine phenylindole; 3-MA = 3-methyladenine. The values are the mean ± SD; n = 3, *P < .05.