Fig. 4. Stimulation of mitochondrial ROS and depletion of mitochondrial lipids in PCBP1^Δhep mice.

A. Elevated ROS production in hepatocytes from PCBP1^Δhep mice. Primary hepatocytes were analyzed for mitochondrial ROS using mitochondrial-specific ROS probe Mitosox. Fluorescence expressed as ratio to mitotracker green to normalize for mitochondrial content. Quantification of fluorescence intensity ratios at right. Analyses were repeated thrice; 25–40 cells were analyzed in each experiment. B. Elevated mRNA levels of mitochondrial ROS marker UCP2 in PCBP1^Δhep livers. C. Depletion of Coenzyme Q and major cardiolipins in PCBP1^Δhep livers. Global lipid analysis of livers was performed. Predominant cardiolipin, tetralinoleoyl (C18:2)₄ cardiolipin is shown. Acylcarnitine, a lipid carrier and marker of impaired mitochondrial lipid metabolism, at right. Data represent mean ± SD. (A,B)* indicates p < 0.05, **p < 0.002 as determined by unpaired t-test. (C) * indicates q < 0.05, ** indicates q < 0.002 as determined by False Discovery Rate (FDR) determined using the two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli, with Q = 5%.