Male littermates were weaned onto 50 ppm iron diet supplemented with either vitamin E (500 IU/gm) or CoQ10 via drinking water (0.5 mg/ml) for 16–18 days. A. Prevention of steatosis with CoQ10. H&E staining of fixed liver tissues. B. Normalization of liver triglycerides and reduction of serum ALT with CoQ10. C. Normalization of Nrf2 target genes MT-1, NQO1 by qPCR using RNA isolated from liver tissue. D. Normalization of mitochondrial stress marker UCP2. E. Normalization of ATP levels. F. Normalization of acylcarnitine and cardiolipin levels. Data represent mean ± SD. * indicates p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns not significant, as determined by 2-way ANOVA with Bonferroni multiple comparison test.