KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal OGR1 | Abcam | ab72500,; RRID:AB_1523738 |
| Rabbit polyclonal OGR1 | LSBio | LS-A1194; RRID:AB_591626 |
| Rabbit polyclonal PLIN2/ADFP | Abcam | ab78920; RRID:AB_2040415 |
| Rabbit monoclonal LC3B | CST | 3868S; RRID:AB_2137707 |
| Rabbit monoclonal phospho AKT S473 | CST | 4060; RRID:AB_2315049 |
| Rabbit polyclonal phospho AKT T308 | CST | 9275; RRID:AB_329828 |
| Rabbit polyclonal Total AKT | CST | 9272; RRID:AB_329827 |
| Rabbit monoclonal GAPDH | Abcam | Ab181602;RRID:AB_2630358 |
| Mouse monoclonal Actin | Sigma | #A1978; RRID:AB_476692 |
| Rabbit polyclonal PLIN2 | LSBio | B4850; RRID:AB_10801440 |
| LAMP2/CD107b | Novus | NBP2-22217; RRID:AB_2722697 |
| LAMP2a/Lysosome marker | Abcam | 125,068; RRID:AB_10971511 |
| Goat anti-Mouse IgG (H+L) Alexafluor 647 | Molecular Probes | A32728; RRID:AB_2633277 |
| Goat anti Rabbit Alexafluor 594 | Molecular Probes | A11012; RRID:AB_2534079 |
| Goat anti-Rabbit Alexafluor 647 | Molecular Probes | A21245; RRID:AB_2535813 |
| Goat anti-Rabbit Alexafluor 680 | Molecular Probes | A27042; RRID:AB_2536103 |
| Rabbit polyclonal phospho NFkB | CST | 3033/93H1; RRID:AB_331284 |
| Total NFkB | CST | D14E12; RRID:AB_2799570 |
| Na/K+ATPase | Abcam | ab76020; RRID:AB_1310695 |
| Rabbit monoclonal Fatty acid Synthase | CST | 3180; RRID:AB_2100796 |
| Rabbit monoclonal ACSS2/AceCS1 | CST | 3658; RRID:AB_2222710 |
| Rabbit polyclonal ACLY | CST | 4332; RRID:AB_2223744 |
| Rabbit monoclonal TFEB | CST | 37785;RRID:AB_2799119 |
| Rabbit monoclonal ACC | CST | 3676; RRID:AB_2219397 |
| Rabbit monoclonal PERK (D11A8) | CST | 5683; RRID:AB_10841299 |
| Rabbit polyclonal Phospho IRE1α (S724) | Abcam | ab124945; RRID:AB_11001365 |
| Donkey Anti-Rabbit IgG Peroxidase conjugated | Pierce | PI-31458; RRID:AB_228213 |
| Donkey Anti-Mouse IgG Peroxidase conjugated | Pierce | PI-31450; RRID:AB_2534691 |
| Bacterial and virus strains | ||
| lentiCRISPR V2 | Addgene | 52961 |
| Stbl3 competent E. coli cells | Invitrogen | C737303 |
| DH5α Competent E. coli cells | Invitrogen | 18258012 |
| Biological samples | ||
| Breast Tissue Micro Array | Moffitt Cancer Center | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| TOFA | Cayman Chemicals | 10005263 |
| C75 | Cayman Chemicals | 191282-48-1 |
| Etomoxir | Cayman Chemicals | 11969 |
| GDC0941 | Selleck Chemicals | Catalog No. S1065 |
| MK2206 | Selleck Chemicals | Catalog No. S1078 |
| Chloroquine diphosphate | Sigma | C6628 |
| Bafilomycin A1 | Cayman Chemicals | Item No.11038 |
| PLC inhibitor U-73122 hydrate | Sigma | U6756 |
| Edelfosine | Tocris | Cat.No.3022 |
| Ogerin | Sigma | SML1482 |
| DMEM, High Glucose | Gibco | 11965-092 |
| DMEM w/o L-glutamine, leucine, sodium pyruvate | MP Biomedicals | 091642149 |
| DMEM, no glucose | Gibco | 11966-025 |
| DMEM without Leucine | CRYSTALGEN INC | NC0565703 |
| PIPES | Sigma | P1851 |
| HEPES | SIGMA | H4034 |
| Delipidated serum | Gemini Bioproducts | 900-123 |
| 13C5 L-GLUTAMINE | Cambridge Isotope | 1822-H-0.1 |
| D-GLUCOSE (U-13C6, 99%) | Cambridge Isotope | CLM-1396-5 |
| L-Leucine 3-13C | Sigma | 604828 |
| SODIUM ACETATE(2-13C) | Cambridge Isotope Lab | CLM-381-5 |
| SODIUM L-LACTATE-13C3 SOLUTION | Sigma | 485926 |
| Fugene | Promega | PR-E2311 |
| SYBR Green (Sso Advanced) | Biorad | 172-5271 |
| iScript cDNA synthesis Kit | Biorad | 1708891 |
| Power SYBR green | ABI | 4368702 |
| MTT | Sigma | M2128 |
| Oligofectamin | Invitrogen | 12252-011 |
| Lipofectamine RNAiMAX | Invitrogen | 13778075 |
| Thiazolyl Blue MTT | Sigma | M2128-500MG |
| Mounting medium with DAPI | Vector Labs | H-1200 |
| Glass chamber slides | LabTek | 12-565-8 |
| Normal Goat Serum | Fisher | PI-31873 |
| Tricine | Sigma | T0377 |
| Lysotracker Deep Red | Invitrogen | L12492 |
| Critical commercial assays | ||
| QIAamp DNA Mini Kit | Qiagen | 51304 |
| RNeasy Plus Mini Kit | Qiagen | 74134 |
| LD Fluorescence Assay Kit | Cayman Chemicals | 500001 |
| iScript cDNA synthesis Kit | Biorad | 1708891 |
| SYBR Green (Sso Advanced) | Biorad | 172-5271 |
| Deposited data | ||
| Lipidomics Data | This paper, https://zenodo.org/record/6449175 | Zenodo Data: https://doi.org/10.5281/zenodo.6449175 |
| Experimental models: Cell lines | ||
| Human: MCF7 | ATCC/PSON | N/A |
| Human: T47D | ATCC/PSON | N/A |
| Human: MDA-MB-231 | Robert Gillies’s Lab, Moffitt Cancer Center | N/A |
| Experimental models: Organisms/strains | ||
| Mouse: 6-8 weeks old Female NSG | Jackson Labs | N/A |
| Oligonucleotides | ||
| EGFP-gRNA-OligoF-5′ CACCG GGGCGAGGAGCTGTTCACCG | This paper | N/A |
| EGFP-gRNA-OligoR -5′ AAAC CGGTGAACAGCTCCTCGCCCC | This paper | N/A |
| OGR1-gRNA-OligoF1-5′ CACCG GGTGGTCTATGTTACCGTGC | This paper | N/A |
| OGR1-gRNA-OligoR1-5′ AAACGC ACGGTAACATAGACCACCC | This paper | N/A |
| OGR13′gRNA-OligoF-CACCGT CACGTGGAGCCACCCGCGG | This paper | N/A |
| OGR13′gRNA-R-AAACCCG CGGGTGGCTCCACGTGAC | This paper | N/A |
| TDAG8-gRNA-OligoF1-CACCGG TCAGCATTCCAGCCAATAT | This paper | N/A |
| TDAG8-gRNA-OligoR1-AAAC ATATTGGCTGGAATGCTGACC | This paper | N/A |
| TDAG8-3′ gRNA-OligoF-CACCGTCATTTAATAAAACGCAGGG | This paper | N/A |
| TDAG83′gRNA-OligoR- AAAC CCCTGCGTTTTATTAAATGAC | This paper | N/A |
| Recombinant DNA | ||
| lentiCRISPR V2 | Addgene | 52961 |
| lentiCRISPR constructs with gRNA inserts listed above | This paper | N/A |
| OGR1(Myc-DDK) tag | Origene | CAT#: RC229950 |
| pCMV6-Entry | Origene | CAT#: PS100001 |
| Software and algorithms | ||
| Polly El-MAVEN | El-MAVEN v0.8.0 | N/A |
| MSConvert | He et al., 2015 | https://proteowizard.sourceforge.io/download.html |
| LAS AF software version 1.6.0 build 1016 | Leica Microsystems, Germany | N/A |
| Definiens Tissue Studio v4.7 suite | Definiens Inc, Germany | N/A |
| Incucyte S3 | Select Science | N/A |
| Other | ||
| GPR68/OGR1siRNA | Ambion | Cat. #4427037 |
| TDAG8/GPR65siRNA | Ambion | s16100 |
| Silencer Negative Control siRNA | Ambion | AM4611 |