Skip to main content
. 2022 Apr 30;11(5):897. doi: 10.3390/antiox11050897

Figure 2.

Figure 2

Glycine-N-methyltransferase (GNMT) expression is decreased in DILI by the enhanced expression of miR-873-5p. (A) Western blot of GNMT, loading control and respective quantification in primary hepatocytes cultured for 1, 3 and 6 h with 10 mM acetaminophen (APAP); (B) Western blot of GNMT and Methionine adenosyltransferase I/III (MATI/III) and relative expression of (C) mRNA from methionine and folates cycles enzymes in liver from mice administered 360 mg/kg APAP for 48 h. miR-873-5p expression in (D) primary hepatocytes cultured for 1, 3 and 6 h with APAP, (E) Liver from mice administered 360 mg/kg APAP for 48 h and (F) serum from drug-induced liver injury patients (DILI) compared to a control group or healthy individuals. (G) Correlation between levels in serum of miR-873-5p and aspartate aminotransferase (AST), alanine. aminotransferase (ALT), alkaline phosphatase (ALP) and total bilirubin (TBL) from DILI patients compared to healthy individuals. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 are shown.