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. 2022 May 10;9(5):203. doi: 10.3390/bioengineering9050203

Figure 1.

Figure 1

Methodology to create dcECM extract and tissue culture surface coating. (A) Representative macroscopic and Toluidine Blue (TB) image of control hypertrophic costal cartilage and dcECM (decellularised costal cartilage extracellular matrix); Scale Bar = 50 µm. (B) Methodology used to create dcECM extract: (1) Digestion solution (1 mg/mL Pepsin in 0.01 M HCL); (2) Sterile freeze-dried dcECM granules are digested at a concentration of 10 mg/mL; (3) extract after 96 h. (C) Brightfield images of tissue culture plastic coated with non-sterilised, (D) UV sterilised, and (E) autoclaved dcECM extract; Scale Bar = 100 µm.