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. 2022 Apr 30;11(5):891. doi: 10.3390/antiox11050891

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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SEC profiles and SDS-PAGE images (insets) of recombinant UPOs and variants (the positions of standards are indicated). (A) rMroUPO (black) and its C227A variant (blue). (B) rCviUPO (black) and its C235A (blue), K228stop (green), and P201stop (red) variants. SEC was performed with a Superdex 75 column and isocratic elution with 10 mM Tris (pH 7.4) containing 0.15 M NaCl (given the low production of the C227A variant, its absorbance in A is multiplied by a factor of 15). SDS-PAGE was performed with 0.1% SDS containing 1% mercaptoethanol, and same protein amount (~1.7 µg) was loaded in each case.