Inhibition of RBOH-dependent signaling or BRs during recovery after CS-ACC affected the expression of CsRBOH1 (A), CsRBOH2 (B), CsRBOH3 (C), CsRBOH4 (D), CsRBOH5 (E), CsRBOH6 (F), CsRBOH7 (G), CsRBOH8 (H) and CsRBOH9 (I), respectively. CK: control, cucumber seedlings with no acclimation and at 25 °C during recovery; CS-ACC: cucumber seedlings were treated with a cold stress regime of 10 °C for 1 h, 25 °C for 1.5 h, and 1 °C for 3 h; CS-ACC-DPI: diphenyleneiodonium (DPI) was sprayed onto the seedlings during recovery after CS-ACC; CS-ACC-BRZ: brassinozole (BRZ) was sprayed onto the seedlings during recovery after CS-ACC; R: recovery, 25 °C. The different letters indicate significant differences as assessed by Turkey HSD test (p < 0.05; n ≥ 4).