The cold tolerance (A) and CII (B) of cucumber seedlings tested by a tester cold stress (1 °C, 24 h) following 48 h of recovery after CS-ACC or CS-ACC-DPI, and then placed in recovery for a period (25 °C/18 °C, day/night, 7 days). (A): a schematic diagram and cold tolerance of CS-ACC with LR and CS-ACC-DPI with LR. CS-ACC: cucumber seedlings were treated with a cold stress regime of 10 °C for 1 h, 25 °C for 1.5 h, and 1 °C for 3 h; CS-ACC-DPI: DPI was sprayed onto the seedlings during recovery after CS-ACC. (B): CII of cucumber seedlings. (C–F): the H2O2 content, NADPH oxidase activity, electrolytic leakage, and proline content during recovery after CS-ACC or CS-ACC-DPI, respectively. CS-ACC: cold stress acclimation; LR: long recovery, 25 °C. The different letters indicate significant differences as assessed by the Turkey HSD test (p < 0.05; n ≥ 3).