Table 1.
List of primers used.
| Cell Line | Gene Name | Forward Sequence | Reverse Sequence | Accession Number (RefSeqID) |
|---|---|---|---|---|
| 16HBE | SIRT1 | AAGGAAAACTACTTCGCAAC | GGAACCATGACACTGAATTATC | NM_001142498 |
| CDKN1A(p21) | CAGCATGACAGATTTCTACC | CAGGGTATGTACATGAGGAG | NM_000389 | |
| GPX2 | AATTTGGACATCAGCTGC | GGCTGCTCTTCAAGATTTAG | NM_000852 | |
| IL-1β | GCCTCAAGGAAAAGAATCTG | GGATCTACACTCTCCAGG | NM_000576 | |
| IL-6 | GCAGAAAAAGGCAAAGAATC | CTACATTTGCCGAAGAGC | NM_000600 | |
| TNF-α | AGGCAGTCAGATCATCTTC | TTATCTCTCAGCTCCACG | NM_000594 | |
| NQO-1 | AGTATCCACAATAGCTGACG | TTTGTGGGTCTGTAGAAATG | NM_000903 | |
| GCLC | TTATTAGAGACCCACTGACAC | TTCTCAAAATGGTCAGACTC | NM_001197115 | |
| PTGS2 (COX-2) | AAGCAGGCTAATACTGATAGG | TGTTGAAAAGTAGTTCTGGG | NM_000963 | |
| 5-LOX | AAATGCCACAAGGATTTACC | ATCGCTTTGGAGTAATTCAG | NM_000698 | |
| GAPDH | TCGGAGTCAACGGATTTG | CAACAATATCCACTTTACCAGAG | NM_002046 | |
| RAW264.7 | Gclc | CGACCAATGGAGGTGCAGTTA | AACCTTGGACAGCGGAATGA | NM_010295.2 |
| Nqo1 | GTAGCGGCTCCATGTACTCTC | AGGATGCCACTCTGAATCGG | NM_008706.5 | |
| Tnf-α | TCTGTCTACTGAACTTCGGGGTGA | TTGTCTTTGAGATCCATGCCGTT | NM_013693.3 | |
| IL-6 | AGAAAACAATCTGAAACTTCCAGAGAT | GAAGACCAGAGGAAATTTTCAATAGG | NM_031168 | |
| Gpx2 | ACC AGTTCGGACATCAGGAG | CCC AGGTCGGACATACTTGA | NM_030677 | |
| IL-1β | TGGGATCCTCTCCAGCCAAGC | AGCCCTTCATCTTTTGGGGTCCG | NM_008361 | |
|
Ptgs2
(Cox-2) |
ACTCATAGGAGAGACTATCAAG | GAGTGTGTTGAATTCAGAGG | NM_011198 | |
|
Alox5
(5-Lox) |
CAGGAAGGGAACATTTTCATC | AGGAAGATTGGGTTACTCTC | NM_009662 | |
| Hprt | AGGCCAGACTTTGTTGGATTTGAA | CAACTTGCGCTCATCTTAGGCTTT | NM_013556.2 |