TABLE 2.
Clone | Classification | Relative activity ofa:
|
Apparent molecular size (kDa) | ||
---|---|---|---|---|---|
MUF-NAG | MUF-diNAG | MUF-triNAG | |||
pJAM2 | ND | ND | + | ND | — |
pJAM4 | Chitobiosidase | 0 | ++ | 0 | — |
pJAM5 | Chitobiosidase | 0 | ++ | 0 | 250 |
pJAM6 | Exochitinase | ++ | + | + | 30 to 250b |
pJAM9 | Exochitinase | + | + | + | 98 |
pJAM17 | ND | ND | + | ND | — |
pJAM19 | Endochitinase | 0 | + | + | 98 |
pJAM24 | ND | ND | + | ND | — |
pJAM28 | ND | ND | + | ND | — |
The hydrolytic activities of protein extracts measured with various fluorogenic N-acetylglucosamine oligomers and polyacrylamide gels containing glycol chitin as indicated are shown. Relative activities: ND, not determined; 0, background activity; +, 2- to 10-fold above background; ++, 10- to 50-fold above background; +++, 50- to 100-fold above background. —, No hydrolysis.
The clearing zone appeared as a smear from 30 to 250 kDa.