Figure 2.

Effects of curine and guattegaumerine on P. aeruginosa growth. A total of 100 µL LB medium containing 1 × 10⁵ bacteria of strain IP104116 and increasing concentrations of curine or guattegaumerine were added in each well of 96-well plates. After 24 h of incubation at 37 °C in a wet chamber, growth was estimated by measuring the absorbance at 595 nm and was compared to the control without curine or guattegaumerine and plotted against concentration in alkaloids in the corresponding well. Experiments were repeated at least four times for each strain of P. aeruginosa used in this study.