Table 3.
Effects of verapamil on the sensitivity of P. aeruginosa PAO1 and its mutants to carbenicillin and novobiocin.
| Carbenicillin IC 50 (µg/mL) | Novobiocin IC 50 (µg/mL) | ||||
|---|---|---|---|---|---|
| Strain | Gene | None | Verapamil | None | Verapamil |
| Mean ± SD | Mean ± SD, p-Value | Mean ± SD | Mean ± SD, p-Value | ||
| PAO1 Washigton | 29 ± 3.5 | 21 ± 2, (p = 0.05) | 193 ± 3 | 166 ± 2, (p = 0.002) | |
| PW2567 | PA0860 | nd | nd | 267 ± 12 | 231 ± 9, (p = 0.015) |
| PW8020 | PA4143 | 42 ± 2 | 23 ± 1, (p = 0.08) | 180 ± 11.5 | 158.5 ± 6.5, (p= 0.002) |
| PW6408 | PA3228 | 55 ± 4 | 39 ± 4, (p = 0.09) | 249 ± 8 | 210 ± 5.5, (p = 0.09) |
| PW3010 | PA1113 | 54 ± 2 | 54 ± 1.5, (p = 0.01) | 146.5 ± 3.5 | 127 ± 3.5, (p = 0.10) |
The average value of the IC50 at the plateau (calculated from each curve, as presented in Figure 4) for carbenicillin or novobiocin in the presence of verapamil was reported (right columns) and compared to the value of IC50 in the absence of verapamil (left columns). The results presented in this table are the mean values of at least four independent experiments. p-values (p) were obtained using Fisher’s exact test.