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. 2022 Apr 21;11(5):811. doi: 10.3390/antiox11050811

Table 1.

List of the primers used to quantify the gene expression levels.

Name Gene Identifier Description Forward Primer (5′ to 3′) Reverse Primer (5′ to 3′)
KfACTIN Kaladp0071s0282 As reference gene TTCGAGACCTTCAATGCTCCT GATGGCTGGAAAAGCACCTCA
KfPHYA Kaladp0034s0172 Phytochrome A, flowering promoted gene [Kalanchoe fedtschenkoi] TTCAGCCATTTGGGTGTTTGT CGTATCACACAGCTTGTCCAG
KfPHYB Kaladp0039s0298 Phytochrome B, flowering suppressor gene [Kalanchoe fedtschenkoi] CACATGCTCTCTCTGACTCCC AAAAGCTGAATGTCCCCTCCA
KfCRY1 Kaladp0071s0308 Cryptochrome 1, flowering promoted gene [Kalanchoe fedtschenkoi] TCTTGGCGCCAGTTTCATCA TGTCAGCATTGCTCCATCCA
KfFT Kaladp0099s0141 PROTEIN FLOWERING LOCUS T (FT), regulation of flower development [Kalanchoe fedtschenkoi] AGGGAAGTTGCAAATGCGTG GGAAGTTCTGACGCCATCCC
KfFPF-1 Kaladp1115s0001 FLOWERING-PROMOTING FACTOR 1-like PROTEIN 1-related [Kalanchoe fedtschenkoi] AGCGCTGATCAAAACGGTAGA GAAAAATCGCGAGGAAGGGA
KfPEF-4 Kaladp0059s0037 PROTEIN EARLY FLOWERING 4 [Kalanchoe fedtschenkoi] GGCGGCGGAGATATTTGTGA TGGCAGCACGATGATGAAAG