Figure 4.

Time courses of NADPH consumption and arsenous product accumulation. The reaction mixture initially contained 142 μM NADPH, 50 μM FMN, 159 μM PhAsO(OH)₂ and 9 nM ArsH in 0.1 M sodium phosphate buffer (pH 7.0, 30 °C). Oxidation of NADH was continuously monitored at 340 nm (solid line) while samples were taken for HPLC analysis of the phenylarsonous acid levels (points lying on the dotted line). Vertical bars denote standard deviations from triplicate samples.