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. 2022 May 3;11(5):902. doi: 10.3390/antiox11050902

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Electrophoretic shift assay with a 27-bp arsR promoter DNA fragments with ArsR proteins. FAM-labeled DNA fragments were incubated with or without purified ArsR proteins for 30 min at room temperature. The mixtures were electrophoresed on 3% agarose gels at 7 °C with ethidium bromide and visualized by UV-transilluminator. When either 10 μM PDNA1 (lane 1) or PDNA2 (lane 6) was incubated with the recombinant protein 10 μM ArsR1 (lane 2 and 3) or ArsR2 (lane 7 and 8), retardation due to DNA-protein complex formation was observed. The retardation was almost suppressed in the presence of 1 mM sodium arsenite (lanes 4 and 9) or 1 mM diamide (lanes 5 and 10) for both complexes. M means the marker and it was 100 bp DNA ladder from Promega.