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. 2022 May 3;11(5):902. doi: 10.3390/antiox11050902

Figure 9.

Figure 9

ArsR binding to DNA labelled by fluorescence probe. Titration was conducted in 10 mM MOPS/0.1 M NaCl/15% glycerol, pH 7.0 at 30 °C. Panel (A), 25 nM concentration of PDNA1 (•) or PDNA2 (◦) was titrated with the indicated concentrations of the recombinant protein ArsR1 protein. Panel (B), 25 nM concentration of PDNA1 (▫) or PDNA2 (▪) was titrated with the indicated concentrations of the recombinant ArsR2 protein. Solid lines represent the best fit of the data to Equation (5). The fitted parameters are presented in the main text. Each data point is a mean of ten determinations ± SD.