Skip to main content
. 2022 May 26;17(1):978–990. doi: 10.1515/med-2022-0487

Figure 3.

Figure 3

Construction of Rack1 overexpression plasmid and siRNA. Rack1 was overexpressed or silenced by Rack1(+) plasmid or Rack1 siRNAs by (a) qRT-PCR and (b) western blot. The result showed that the expression of Rack1 was significantly increased in mesangial cells transfected with Rack1 overexpression plasmid Rack1(+) in the L-MC group compared with that in cells transfected with empty plasmid pcDNA3.1 and untreated cells. Moreover, the result showed that the expression of Rack1 was decreased in mesangial cells transfected with Rack1 siRNAs (No. 2 and No. 3) in the H-MC group compared with that in cells transfected with the siRNA control and untreated cells by qRT-PCR. Data showed the effect of No. 2 was best. The data are representative of three independent experiments and presented as mean ± SD. **p < 0.01; *p < 0.05; NS, no significant.