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. 2022 May 26;17(1):978–990. doi: 10.1515/med-2022-0487

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© 2022 Keqian Wu et al., published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

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Rack1 promotes the expression of inflammatory factors in mesangial cells under high glucose conditions. (a) The expression of Mcp-1 was regulated by Rack1 by qRT-PCR. The result showed that the expression of Mcp-1 was significantly increased in mesangial cells transfected with Rack1 overexpression plasmid Rack1(+) in the L-MC group compared with that in cells transfected with empty plasmid pcDNA3.1 and untreated cells, while the expression of Mcp-1 was decreased in mesangial cells transfected with siRack1 in the H-MC group compared with that in cells transfected with the siRNA control and untreated cells. The data are representative of three independent experiments. Data are presented as mean ± SD. ^** p < 0.01; NS, not significant. (b) The expression of Tnf-α was regulated by Rack1 by qRT-PCR. The result showed that the expression of Tnf-α was significantly increased in mesangial cells transfected with Rack1 overexpression plasmid Rack1(+) in the L-MC group compared with that in cells transfected with empty plasmid pcDNA3.1 and untreated cells, while the expression of Tnf-α was decreased in mesangial cells transfected with siRack1 in the H-MC group compared with that in cells transfected with the siRNA control and untreated cells. The data are representative of three independent experiments. Data are presented as mean ± SD. ^** p < 0.01;, NS, not significant. (c) The expression of Mcp-1 was regulated by Rack1 by ELISA. The result showed that the expression of Mcp-1 was significantly increased in mesangial cells transfected with Rack1 overexpression plasmid Rack1(+) in the L-MC group compared with that in cells transfected with empty plasmid pcDNA3.1 and untreated cells, while the expression of Mcp-1 was decreased in mesangial cells transfected with siRack1 in the H-MC group compared with that in cells transfected with the siRNA control and untreated cells. The data are representative of three independent experiments. Data are presented as mean ± SD. ^* p < 0.05, ^** p < 0.01; NS, not significant. (d) The expression of Tnf-α was regulated by Rack1 by ELISA. The result showed that the expression of Tnf-α was significantly increased in mesangial cells transfected with Rack1 overexpression plasmid Rack1(+) in the L-MC group compared with that in cells transfected with empty plasmid pcDNA3.1 and untreated cells, while the expression of Tnf-α was decreased in mesangial cells transfected with siRack1 in the H-MC group compared with that in cells transfected with the siRNA control and untreated cells. The data are representative of three independent experiments. Data are presented as mean ± SD. ^* p < 0.05, ^** p < 0.01; NS, not significant.