Figure 4.

Viability of intestinal IPEC-J2 cells. (a) Dose−response curves with different concentrations of Ascophyllum nodosum, Lithotamnium calcareum and the combination of both algae; (b) Viability of IPEC-J2 cells challenged with H₂O₂ 0.5 mM and pre-treated with Ascophyllum nodosum, Lithotamnium calcareum and the combination of both algae; (c) Viability of IPEC-J2 cells challenged with H₂O₂ 1 mM and pre-treated with Ascophyllum nodosum, Lithotamnium calcareum and the combination of both algae. Data are shown as means and standard deviations. ^a–e Means (n = 3) with different superscripts are significantly different (Treatment p < 0.05).