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. 2022 Apr 24;11(5):829. doi: 10.3390/antiox11050829

Figure 2.

Figure 2

Detection of Se levels, selenoenzyme activity and selenoprotein expression in the brains of 7-month-old AD mice upon Se-FA administration. (a) Se levels in the cortex and hippocampus of NTg, 3×Tg-AD and Se-FA-treated mice were measured by atomic fluorescence spectrometry (n = 5 mice). (b,c) The activity of GPx (b) and TrxR (c) in the brains of 3×Tg-AD mice was detected using specific assay kits (n = 3 or 5 mice). (d) Levels of trxR1, SELENOP, GPx4, GPx1 and SELENOR proteins in the cortex and hippocampus of 3×Tg-AD mice were analyzed by immunoblotting. (e,f) Quantitation of the protein levels in d ((e) cortex; (f) hippocampus; n = 3 or 5). α-Tubulin, β-actin or GAPDH was used as a loading control. All the mice were fed diets with Se-FA or a control diet for 12 weeks. All data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 as determined by one-way ANOVA followed by Dunnett’s multiple comparison test.