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. 2022 Apr 24;11(5):829. doi: 10.3390/antiox11050829

Figure 5.

Figure 5

Se-FA inhibits Aβ production and tau hyperphosphorylation in 3×Tg-AD mice. (a) Aβ1-42 levels in the cortex and hippocampus of 7-month-old 3×Tg-AD mice measured by ELISA (n = 3 or 5 mice). (b) Levels of BACE1 and Aβ oligomers were analyzed by immunoblot in the cortex and hippocampus of 3×Tg-AD mice. (c) Quantitation of the protein levels in (b) (n = 3 or 5 mice). (d) Representative total tau (HT7) and pS202-tau (AT8) immunostaining in the CA3 area of the hippocampus of NTg-, 3×Tg-AD- and Se-FA-treated mice (Scale bar: 50 μm). (e) Levels of total tau and p-tau (Ser202 and Ser214/Thr212) in the cortex and hippocampus of experimental mice were analyzed by immunoblotting. (f) Quantitation of the protein levels in e (n = 3 or 5 mice). (g) Levels of PP2A and p-PP2A (Tyr307) in the cortex of 3×Tg-AD mice were analyzed by immunoblotting. (h) Quantitation of the protein levels in g (n = 3 or 5 mice). α-Tubulin, β-actin or GAPDH was used as a loading control. All the mice were fed diets with Se-FA or a control diet for 12 weeks. All data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 as determined by one-way ANOVA followed by Dunnett’s multiple comparison test.