Figure 6.

Se-FA restores synaptic plasticity and improves cognitive ability in 3xTg-AD mice. (a) Levels of PSD95 and synaptophysin were analyzed by immunoblot in the cortex and hippocampus of mice. α-Tubulin was used as a loading control. (b) Quantitation of the protein levels in (a) (n = 3 or 5 mice). (c) Slope of fEPSPs in response to 100-Hz stimulation in the Schaffer collateral CA1 region of the mice. (d) Quantification of the averaged fEPSP slope of the last 10 min in (c) (n = 12 sections, 3 sections per mouse). (e–h) Morris water maze tests were performed in 7-month-old 3×Tg-AD mice (two-way ANOVA followed by Dunnett’s multiple comparison test) (n = 8 or 10 mice). The escape latency time to reach the hidden platform was recorded during the 4-day training (e). Swimming speed was recorded during the probe trial (f). The probe trial was performed 24 and 72 h after the last trial of a hidden platform task. The number of crossings over the original platform area was recorded (g), along with the percentage of search time spent in each quadrant (h). (i,j) The number of arm entries (i) and number of correct alternations (j) in the Y maze test (n = 9 mice). All the mice were fed diets with Se-FA or a control diet for 12 weeks. All data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 as determined by one-way or two-way ANOVA followed by Dunnett’s multiple comparison test.